ANTIGEN AND ANTIBODY CORE
Barbara A. Christy, Ph.D.
CORE DESCRIPTION
The goal of this core facility is to provide custom antibody reagents and related services to investigators of the San Antonio Cancer Institute and the University of Texas Health Science Center at San Antonio. Animals (mice) used in the generation of novel antibody reagents are housed in the animal facility on the first floor of the Institute of Biotechnology, with complete care and veterinary services. Animal rooms are equipped with microisolators housed on racks with a controlled supply of treated water and filtered air.
FUNDING
This resource is funded by the San Antonio Cancer Institute (P30 CA54174) Tyler J. Curiel, Principal Investigator. All users of this core must cite the support of the San Antonio Cancer Institute Cancer Center Support Grant (P30CA54174) in all publications and presentations involving work performed by this Shared Resource.
EQUIPMENT
Equipment available to the Antigen & Antibody core facility include laminar flow hoods, ELISA Plate reader, tissue culture incubators, a liquid nitrogen freezer, a refrigerator/freezer, centrifuges, and other necessary general molecular /cell biology laboratory equipment.
SERVICES
The services offered by the core include the design and production of fusion protein antigens, production and purification of antibody from hybridoma cells, production of mouse or rabbit polyclonal antisera, production of mouse monoclonal antibodies, screening for positive hybridoma supernatants by ELISA, western blotting and/or immunoprecipitation, single-cell cloning of hybridoma cells, adaptation to serum-free media, cryopreservation, and cryostorage.
