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CORE DESCRIPTION

The mission of the Genetic Mouse Models Shared Resource is to provide a centralized resource to the members of the Department of Molecular Medicine/Institute of Biotechnology for the design, generation and evaluation of genetically altered mice. The Genetic Mouse Models Shared Resource provides the following services:

FUNDING

This resource is funded by the Department of Molecular Medicine/Institute of Biotechnology.

EQUIPMENT

Tissue culture: two hoods, two incubators (CO2 water jacketed incubator series II), centrifuge, gene pulser Xcell, NI-150 transilluminator, Nikon SMZ800, Nikon SMZ645, Nikon eclipse TS100, Narishige injector, eppendorph celltram Vario injectior, Bionomic controller, Nikon digital camera DXM1200

SERVICES

Design of genomic alteration. Assistance is provided in protocol design from basic knockouts to a wide-range of more sophisticated approaches such as tissue-specific and conditional knockouts. In addition, this core will provide transgenic services using mouse embryonic stem (ES) cells.

Isolation of ES cells with the desired genomic alteration. DNA vectors are transfected into ES cells and the appropriate selection media is applied. Drug-resistant colonies will be isolated, expanded and replica-plated.

Generation of genetically altered mice (heterozygotes). ES cells with the appropriate genetic modification will be injected into donor blastocysts and transferred into pseudopregnant females. These chimeric mice will be bred to obtain genetically modified heterozygote mice. After genotyping the chimera’s progeny, the genetically modified mice will be delivered to the investigator.

Consultation on analysis of genetically altered mice. Once the genetically altered mice have been established, consultation is offered to investigators on husbandry, genotyping and phenotypic analysis.

Collaborations resulting from this core have been developed with Drs. Jan Vijg, Yousin Suh, Hanna Abboud, Robin Leach, Tom Boyer, Z. Dave Sharp and Babatunde Oyajobi.



 

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