Department of Molecular Medicine

MASS SPECTROMETRY SHARED RESOURCE

 

Pawel Osmulski, Ph.D.

 

The mission of the Mass Spectrometry Core Facility is to provide comprehensive, state-of-the-art, timely, and cost-efficient mass spectrometry support for the qualitative and quantitative analysis of proteins and other biomedically relevant macromolecules. The suitability of mass spectrometry as a technological means to expedite a growing demand for such analyses represents the basis for establishing an on site facility.

Core Director is Dr. Pawel Osmulski.

Core services have thus far been utilized for a variety of research applications, including proteomics, drug discovery, and quality control projects. The great majority of these have involved analyses of protein networks linked to normal biological programs, including transcription regulation, DNA repair, and regulated protein degradation.

The Mass Spectrometry Core Facility encompasses a 200 sq. ft. laboratory and includes the following instrumentation:

- Applied Biosystems Voyager DE-PRO Biospectrometry workstation, consisting of a high performance integrated MALDI-TOF mass spectrometer, a SymBiot I Sample Workstation, and a PS-1 sample handling and data analysis system.

- Thermoquest integrated LC/MS system consisting of a Surveyor HPLC coupled in line with a Finnigan LCQ DUO ion trap mass spectrometer with MS/MS capability.
Presently, we offer users MALDI-TOF services for (1) molecular mass determination of proteins, small molecules, and nucleic acids, and (2) protein identification via peptide mass fingerprinting. These services rely on the use of an Applied Biosystems Voyager DE PRO MALDI-TOF mass spectrometer.

We also established capabilities to offer ESI-MS services to complement our MALDI-TOF based studies. Specifically, ESI-MS permits us to extend our proteomics-based services to include unambiguous protein identification through amino acid sequence analysis and identification of protein post-translational modifications.